Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The level of adiponectin in the serum (A) was measured by ELISA. The level of adiponectin mRNA in ovarial adipose tissues was determined by real-time PCR, and expressed relative to that of β-actin mRNA. Data represent the mean ±SE (n = 6). # p <0.05 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The level of SAA in the serum was measured by ELISA. Data represent the mean ±SE (n = 6). # # # p <0.001 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The level of SAA mRNA in the liver (A) was measured by real-time PCR and expressed relative to that of β-actin mRNA. The level of SAA protein in the liver (B) was measured by ELISA. # # # p <0.001 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The levels of TNFα (A), IL-1β (B), and IL-6 (C) mRNA in the liver were determined by real-time PCR and expressed relative to the level of β-actin mRNA. The level of IL-6 protein in the liver was measured by ELISA (D). Data represent the mean ±SE (n = 6). # p <0.05 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The level of CGRP mRNA in the liver (A) was measured by real-time PCR and expressed relative to that of β-actin mRNA. The level of CGRP protein in the liver (B) was measured by ELISA. # p <0.05 or ## p <0.01 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The level of CGRP mRNA in the hypothalamus (A) was determined by real-time PCR and expressed relative to that of β-actin mRNA. The level of CGRP protein in the hypothalamus (B) was measured by ELISA. Data represent the mean ±SE (n = 6). # p <0.05 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The levels of CGRP mRNA in the epidermis (A) and eye (B) were measured by real-time PCR and expressed relative to the level of β-actin mRNA. The level of CGRP in the serum (C) was measured by ELISA. Data represent the mean ±SE (n = 6). ## p <0.01 indicates values that are significantly different from the UVB (−) group. UVB (−), non-UVB-irradiated mice; UVB (+), UVB-irradiated mice.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Irradiation

Journal: PLoS ONE

Article Title: Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A

doi: 10.1371/journal.pone.0098040

Figure Lengend Snippet: The CGRP signal induced by exposure of the skin to UVB can transfer to the brain and then to the liver, possibly via a neural pathway. Increased CGRP in the liver induces the expression and secretion of SAA via the action of IL-6. In an endocrine manner, SAA in the serum downregulates PPARγ, C/EBPα, C/EBPβ, and aP2 mRNA levels and upregulates IL-6 and MCP-1 mRNA levels in the ovarial adipose tissues. The downregulation of adiponectin expression in the adipose tissues by these factors contributes to the decrease in serum adiponectin. Reduced levels of adiponectin in the serum may impair skin function (Yamane et al . 2010). Thus, the decrease in adiponectin induced by UVB irradiation can have adverse effects on skin function.

Article Snippet: The level of adiponectin, SAA, IL-6, and CGRP proteins in all the samples were determined using the Mouse/Rat Adiponectin ELISA kit (Otsuka Pharm Co., Tokyo, Japan), PHASETM RANGE Mouse SAA ELISA kit (Tri-Delta Diagnostics Inc., Morris Plains, NJ, USA), Mouse IL-6 ELISA Ready-SET-Go! kit (eBioscience Inc., San Diego, CA, USA), and CGRP ELISA kit (USCN Life Science Inc., Huston, TX, USA), respectively, according to the manufacturer's instructions.

Techniques: Expressing, Irradiation

Journal: bioRxiv

Article Title: Allergen-induced dendritic cell migration is controlled through Substance P release by sensory neurons

doi: 10.1101/2020.06.05.136929

Figure Lengend Snippet: ( A ) Wild type mice were i.d. injected with heat inactivated papain (HIP) or papain, skin explants of the injected site were harvested and incubated in serum free media, and supernatant was tested by ELISA for Substance P and CGRP. (A) Dorsal root ganglia were harvested from wild type mice and were left unstimulated with PBS, or stimulated with either HIP or papain. Supernatants were measured by ELISA for Substance P and CGRP release. ( C ) Wild type (WT) or Tac1 -/- mice were i.d. immunized with OVA/Papain and the dLN was harvested 24 hours later to evaluate the percent of activated (PDL2 + ) CD301b + DCs out of total lymph node cells as well as the total number of activated PDL2 + CD301b + DCs per draining lymph node. ( D ) The skin of Kaede mice was photoconverted and i.d. immunized with OVA, OVA/Papain, OVA/CGRP or OVA/Substance P. The dLN was harvested 24 hours after immunization and total percent of skin emigrant Kaede red cells was determined by flow cytometry. Flow cytometry gating scheme of photoconverted and immunized Kaede mice, showing the gates for CD11c + DCs, followed by CD103 + and CD301b + DCs. Percentage of Kaede red CD301b + DCs and Kaede red CD103 + DCs in response to each immunization. ( E and F ) The (E) percentage and (F) total number of CD301b + DCs and CD103 + DCs that originated from the skin of Kaede mice photoconverted and immunized as in (D). Symbols represent individual mice (A, C, E, F) or replicates (B). Bars indicate mean and error bars indicate SEM. Statistical tests: unpaired t test (A, C), ordinary one-way ANOVA with multiple comparisons (B, E, F). * p<0.05, ** p<0.01, *** p<0.001. Data are representative of at least three independent experiments (B, C, D), combined in (A, E, F), with each experiment including 2-4 mice per group.

Article Snippet: Mouse Substance P and CGRP were detected using competitive mouse Substance P and CGRP ELISA kits (both from Cayman Chemical).

Techniques: Injection, Incubation, Enzyme-linked Immunosorbent Assay, Flow Cytometry